| "Woody" Hastings | |
|---|---|
Woody Hastings
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| Born | March 24, 1927 Maryland |
| Residence | United States |
| Citizenship | United States |
| Nationality | American |
| Fields | Bioluminescence, Circadian rhythms |
| Institutions |
Instructor in Biological Sciences Northwestern University 1953-1957 Assistant Professor of Biochemistry University of Illinois 1957-1966 Professor of Biology Harvard University, 1966-1986; Paul C. Mangelsdorf Professor of Natural Sciences Harvard University 1986 - present |
| Alma mater |
Swarthmore College, 1944-1947; B.A. 1947 (Navy V-12 medical officers training program) Princeton University, 1948-1951; M.A. 1950, PhD. 1951 - adviser E. N. Harvey Johns Hopkins University, 1951-1953 Postdoctoral Fellow with W. D. McElroy |
| Doctoral students | David Johnson States[1][2] |
| Known for | Founding circadian biology |
| Notable awards | NATO Senior Fellow in Science, Foundation Curie, Orsay, France, 1977 |
John Woodland “Woody” Hastings, PhD., born March 24, 1927 in Maryland, is a leader in the field of photobiology, especially bioluminescence, and is one of the founders of the field of circadian biology (the study of circadian rhythms, or the sleep-wake cycle).[3] He is the Paul C. Mangelsdorf Professor of Natural Sciences and Professor of Molecular and Cellular Biology at Harvard University.[4][5][6] He has published over 400 papers and co-edited three books.[6]
Hastings research on bioluminescence has principally focused on bacterial luminescence (over 150 papers) and dinoflagellates (over 80 papers).[6] In addition to bacteria and dinoflagellates, he, with his students and colleagues, has published papers on the biochemical and molecular mechanisms of light production in fungi, cnidarians, ctenophores, polychaetes, insects (fireflies and dipterans), ostracod crustaceans, millipedes, tunicates, and fishes with bacterial light organs. His laboratory produced the first evidence for quorum sensing in bacteria,[7] early evidence of the molecular mechanisms of circadian clock regulation in organisms (first using dinoflagellate luminescence and then expanded to other cellular proteins),[3][8][9] and some of the initial studies of energy transfer in green fluorescent proteins (GFP) in cnidarian luminescence.[10][11]
Contents |
Hastings spent his early years in Seaford, Delaware, at age 10 he joined the choir at the Cathedral of St. John the Divine and attended the choir's in-house boarding school, visiting his family during vacations. Hastings moved to Lenox School in Lenox, Massachusetts in 1941 to complete his secondary education and was interested in literature, physica, mathematics, ice hockey and basketball.[12][13]
Throughout his career Hastings has received numerous awards and honors:
1948-1951: Hastings began his graduate studies at Princeton University (Princeton, NJ) in 1948 in the laboratory of E. Newton Harvey, the world leader of luminescence studies at the time, and focused on the role of oxygen in the luminescence of bacteria, fireflies, ostracod crustaceans and fungi. He received his PhD in 1951.
1951-1953: He then joined the lab of William D. McElroy, another student of Harvey’s, at Johns Hopkins University (Baltimore, MD) where he discovered both the stimulatory effects of coenzyme A and gating control by oxygen of firefly luminescence, and that flavin is a substrate in bacterial luminescence.
1953-1957: In 1953 he joined the faculty in the Department of Biological Sciences at Northwestern University (Evanston, IL). In 1954 he began a long collaboration with Beatrice Sweeney, who was then at the Scripps Institution of Oceanography (La Jolla, CA), in elucidating the cellular and biochemical mechanisms of luminescence in the unicellular dinoflagellate Lingulodinium polyedrum (formerly Gonyaulax polyedra). A byproduct of this initial research was their discovery of circadian control of the luminescence.
1957-1966: Hastings next took a faculty position in the Biochemistry Division of the Chemistry Department at the University of Illinois at Urbana–Champaign (Urbana, IL) where he continued his focus on dinoflagellate and bacterial luminescence and dinoflagellate circadian rhythms.
1966–present: Hastings joined the faculty of Harvard University as Professor of Biology in 1966 and where he remains as an emeritus professor. During this period he continued and expanded his studies of circadian rhythms in dinoflagellates and luminescence in bacteria, dinoflagellates and other organisms. He was elected to the National Academy of Sciences in 2003[12] and received the Farrell Prize in Sleep Medicine for his work on circadian rhythms in 2006.[3][14]
For over 50 years he has also had an affiliation with the Marine Biological Laboratory in Woods Hole, MA. He was the director of the Physiology Course there from 1962-.1966, and served as a trustee from 1966-1970.
Luminescent Bacteria: Hastings investigations of luminous bacteria have led not only to basic discoveries of the biochemical mechanisms involved in their light production,[15] the discovery of a flavin to be a substrate in its luciferase reaction,[16] the determination of gene regulation of the luciferases, but also the first evidence for quorum sensing,[17] a form of bacterial communication. In quorum sensing (initially termed autoinduction), the bacteria release into the medium a substance, the autoinducer, that, once the concentration of this substance reaches a critical level, which is a measure of the number of bacteria in a limited area, transcription of specific other genes that had been repressed are turned on. Once the sequenced autoinducer gene was found to occur widely in gram-negative bacteria quorum sensing became accepted in the early 1990s. Now it is known that in many pathogenic bacteria, similar to what happens for the luciferase proteins, there is delayed production of toxins, which serve to greatly augment their pathogenicity. By curtailing their toxin output until the bacterial populations are substantial these bacteria can generate massive quantities of toxin quickly and thereby swamp the defenses of the host.
Luminescent Dinoflagellates: Beginning in 1954 at Northwestern University Hastings and his students and colleagues have been studying cellular and molecular aspects of bioluminescence in dinoflagellates [especially Lingulodinium polyedrum (formerly Gonyaulax polyedra)]. They have been elucidating the structures of the luciferins and luciferases,[18] the organization and regulation of their associated genes, temporal control mechanisms,[19] and the actual subcellular identity and location of the light emitting elements, which they termed scintillons.[20] They demonstrated that the reaction is controlled by a drop in pH when an action potential leads to the entry of protons via voltage-activated membrane channels in the scintillons.[21] Through immunolocalization studies the Hastings lab showed that scintillons are small peripheral vesicles (0.4 μm) that contain both the luciferase and the luciferin-binding protein.[22] Recently their lab has found that the luciferase gene in Lingulodinium polyedrum and other closely related species contains three homologous and contiguous repeated sequences in a kind of “three-ring circus with the same act in all three.”[23] However, another luminescent, but heterotrophic, dinoflagellate, Noctiluca scintillans, has but a single protein, which appears to possess both catalytic and substrate binding properties in a single, rather than separate proteins.
Dinoflagellate Circadian Rhythms: Using Lingulodinium polyedrum as a model, Hastings has spear-headed our understanding of the molecular mechanisms involved in control of circadian rhythms,[24] which in humans are involved in sleep, jet-lag and other daily activities. His lab has shown that the rhythm of bioluminescence involves a daily synthesis and destruction of proteins.[25] Because the mRNAs that code for these proteins remain unchanged from day to night, the synthesis of these proteins is controlled at the translational level.[26] This work has now been expanded to other proteins in the cell. On the other hand, short pulses of inhibitors of synthesis of these proteins results in phase shifts of the circadian rhythm, either delays or advances, depending when the pulse is administered.[27] At still another level, protein phosphorylation inhibitors also influence the period of the rhythm.[28]
Other luminescent systems: Early in his career Hastings developed techniques to quantify the level of oxygen required in a luminescent reaction for several different species including bacteria, fungi, fireflies and ostracod crustaceans.[29] This work showed that oxygen gating is the mechanism for firefly flashing.[30] In other work when he was in the McElroy lab he examined the basic biochemical mechanism of firefly luciferase and demonstrated that coenzyme A stimulates light emission.[31] His lab first demonstrated that the green in vivo coelenterate bioluminescence occurs because of energy transfer from the luminescent molecule (aequorin), which alone emits blue light, to a secondary green emitter which they termed green fluorescent protein (GFP).[10] Once characterized and cloned, GFP has become a crucial molecule used as a reporter and tagging tool for studying gene activation and developmental patterns.[11] Osamu Shimomura, Martin Chalfie and Roger Tsien received the Nobel Prize in Chemistry in 2008 for their work on this remarkable molecule.
Selected publications: